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Ashikaga Yoshiharu (足利 義晴; April 2, 1511 – May 20, 1550) was the twelfth shōgun of the Ashikaga shogunate from 1521 through 1546 during the late Muromachi period of Japan. He was the son of the eleventh shōgun Ashikaga Yoshizumi.

From a western perspective, Yoshiharu is significant, as he was shōgun when the first contact of Japan with the European West took place in 1543. A Portuguese ship, blown off its course to China, landed in Japan. In 1526, Yoshiharu invited archers from neighbouring provinces to come to the capital for an archery contest.